DNA Polymerase Fidelity: Beyond Right and Wrong
نویسندگان
چکیده
منابع مشابه
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In a recent collaborative study, Thomas A. Kunkel, director of the NIEHS Environmental Biology Program, NIEHS deputy director Samuel H. Wilson, and Roger Woodgate, an investigator at the National Institute of Child Health and Human Development, report novel findings about DNA repair and genome stability in humans. Woodgate's laboratory recently discovered and purified polymerase iota (pol ι), o...
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High-fidelity DNA synthesis conditions are those that exploit the inherent ability of polymerases to discriminate against errors. This review has described several experimental approaches for controlling the fidelity of enzymatic DNA amplification. One of the most important parameters to consider is the choice of which polymerase to use in PCR. As demonstrated by the data in Tables 2 and 3, hig...
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ژورنال
عنوان ژورنال: Structure
سال: 2016
ISSN: 0969-2126
DOI: 10.1016/j.str.2016.10.003